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human oa chondrocytes (Cell Applications Inc)

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Cell Applications Inc human oa chondrocytes
Human Oa Chondrocytes, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 94/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+oa+chondrocytes/Human+Chondrocytes%3A+HC/pm41689136-68-0-4
Average 94 stars, based on 63 article reviews

human oa chondrocytes - by Bioz Stars, 2026-10

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Article Title: Extracellular vesicles from human adipose-derived stem cells relieve pain and inflammation in a rat model of knee osteoarthritis
Article Snippet: EVs were labeled with PKH67 dye (Sigma, USA) and filtered through EV spin columns (MWCO 3000 Da; Invitrogen, USA). .. Human OA chondrocytes (HC-OA; Cell Applications, USA) were derived from human articular cartilage obtained from donors diagnosed with osteoarthritis, as defined by the vendor. .. HC-OA were seeded (1 × 10 5 cells/dish) and incubated with 1 × 10 8 particles/mL of labeled EVs for 12 h. After 4’,6-diamidino-2-phenylindole (DAPI) nuclei staining (Invitrogen, USA), cellular uptake was visualized by confocal laser scanning microscopy (CLSM; Zeiss, Germany).

Article Title: Extracellular vesicles from human adipose-derived stem cells relieve pain and inflammation in a rat model of knee osteoarthritis.
Article Snippet: EVs were labeled with PKH67 dye (Sigma, USA) and filtered through EV spin columns (MWCO 3000 Da; Invitrogen, USA). .. Human OA chondrocytes (HC-OA; Cell Applications, USA) were derived from human articular cartilage obtained from donors diagnosed with osteoarthritis, as defined by the vendor. .. HC-OA were seeded (1 × 105 cells/dish) and incubated with 1 × 108 particles/ mL of labeled EVs for 12 h. After 4’,6-diamidino-2-phenylindole (DAPI) nuclei staining (Invitrogen, USA), cellular uptake was visualized by confocal laser scanning microscopy (CLSM; Zeiss, Germany). hASC-EV treatment in IL-1β-stimulated human OA chondrocytes HC-OAs were seeded (2 × 105 cells/well in 6-well plates) and incubated in growth medium (GM) for 24 h. Cells were stimulated with recombinant human IL-1β (10 ng/ mL; R&D Systems, USA) with or without hASC-EVs (5 × 107, 1 × 108 particles/mL) for 48 h. Quantitative polymerase chain reaction (qPCR) Total RNA was extracted using the RNA-spinTM total RNA extraction kit (iNtRON Biotechnology, Korea) and cDNA was synthesized using the RT2 first strand kit (Qiagen, Germany). mRNA levels of TRPA1, cyclooxygenase-2 (COX-2), MMP-2, MMP-3, MMP-9, and glyceraldehyde3-phosphate dehydrogenase (GAPDH) were quantified using gene-specific primers (Qiagen, Germany) and a Stratagene Mx3000P system (Agilent Technologies, USA).

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